AbYlink™: A template mediated regio-selective labeling method for preclinical imaging of therapeutic antibodies

Presented at the World ADC 2022

Léo Marx², Viktoriia Postupalenko¹, David Viertl³, Natalia Gasilova⁴, Mathilde Pantin², Nadège Gsponer¹, Alexandre Johannsen¹, Thibaut Denoel³, Gerrit Hagens¹, Jean-Manuel Segura¹, Frédéric Lévy⁵, Patrick Garrouste², John Prior³, Margret Schottelius³, Niklaus Schaefer³ and Origène Nyanguile¹

¹HES-SO Valais-Wallis, Institute of Life Technologies, Sion​, SWITZERLAND
²Debiopharm Research and Manufacturing, Martigny, SWITZERLAND
³Nuclear Medicine and Molecular Imaging, Lausanne University Hospital, Lausanne, SWITZERLAND
⁴EPFL Valais-Wallis, EPFL ISIC-GE-VS, Sion, SWITZERLAND
⁵Debiopharm, Lausanne, SWITZERLAND

Summary

We present a new regio-specific conjugation method of payloads to monoclonal antibodies based on an affinity peptide, which guides a reactive payload toward the Fc region of the antibody. The method was initially developed for the conjugation of small metal chelators and fluorophores of high polarity and it has been extended to the conjugation of cytotoxic payloads.